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intercept (tbs) blocking buffer  (LI-COR)


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    Structured Review

    LI-COR intercept (tbs) blocking buffer
    Intercept (Tbs) Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 7506 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tbs+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/custom%40927-60001%4042389555
    Average 98 stars, based on 7506 article reviews
    intercept (tbs) blocking buffer - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: A Borophosphate Glass Doped with Cobalt Oxide Improves Skeletal Muscle Structure and Function in Myopathic Mice
    Article Snippet: Samples were loaded on 4–20% gradient Criterion TGX Midi-Protein gels (Bio-Rad, Hercules, CA, USA) for electrophoresis and transferred to LF-PVDF membranes (Millipore, Burlington, MA, USA). .. Following transfer, membranes were blocked in either 5% milk solution or Intercept TBS Blocking Buffer (Li-Cor Biotechnology, Lincoln, NE, USA) for 1 h at room temperature; followed by incubation with primary antibodies overnight at 4 °C. .. Membranes were exposed to either IRDye 800CW Goat anti-Mouse IgG (RRID: AB_621842, Cat.# 926-32210) or IRDye 800CW Goat anti-Rabbit IgG secondary antibodies (RRID: AB_621843, Cat.# 926-32211) (Li-Cor Biotechnology, Lincoln, NE, USA) for 30–60 min depending on the protein of interest.

    Article Title: AI platform for CRISPR functional mapping and function-based drug design
    Article Snippet: Protein was transferred using Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs (BioRad, 1704156). .. The PVDF membrane was blocked for 40 minutes with a 1:1 ratio of 1x TBS (ThermoFisher, PI28360) and Intercept TBS Blocking Buffer (LICOR, 927-60001). .. After blocking, 1μg/mL of NLRP3 (Invitrogen, PA579740) and 1:6000 GAPDH (Cell Signaling Technologies, 2118) antibody was added and kept at 4C overnight with gentle shaking.

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. The fixed cells were quenched with 50 mM NH 4 Cl for 1 h and permeabilized with PBS containing 0.1% Triton X-100 for 15 min, prior to blocking with TBS Blocking Buffer (LI-COR Bioscience) for 1 h at room temperature. .. For arginase detection, a rabbit anti-arginase was used as primary antibody [21] (1:500 in blocking buffer) for 1 h, followed by an anti-rabbit AlexaFluor 546 (Life Technologies) (1:500) as secondary antibody.

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [ ], anti-SODB (1:500) [ ], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [ ] antibodies diluted in TBS Blocking Buffer (LI-COR Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Article Title: GAP mimetic activity of pan-Ras TCI daraxonrasib synergizes with K-Ras Switch-II pocket inhibition.
    Article Snippet: Proteins were transferred onto 0.2 μM nitrocellulose membranes using a semidry transfer device (Invitrogen iBlot 3). .. Membranes were blocked using Intercept TBS Blocking Buffer (LICOR) for 15 min at room temperature. .. Primary antibody incubation was performed in the presence of the indicated antibodies diluted in Intercept antibody diluent (LICOR) for at least 24 h at 4 °C.

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis
    Article Snippet: .. The fixed cells were quenched with 50 mM NH 4 Cl for 1 h and permeabilized with PBS containing 0.1% Triton X-100 for 15 min, prior to blocking with TBS Blocking Buffer (LI-COR Bioscience) for 1 h at room temperature. .. For arginase detection, a rabbit anti-arginase was used as primary antibody [ ] (1:500 in blocking buffer) for 1 h, followed by an anti-rabbit AlexaFluor 546 (Life Technologies) (1:500) as secondary antibody.

    Article Title: Methods of reducing neuroinflammation
    Article Snippet: Gels were transferred to PVDF membranes (Millipore Immobilon FL PVDF Membrane, Li-Cor, P/N: 926-32099) in Invitrogen NuPage Transfer Buffer (ThermoFisher Scientific, Catalog No. NP0006) for 1 hour at room temperature. .. After transfer, membranes were rinsed three times for ten minutes each in Li-Cor Intercept TBS Blocking Buffer (Li-Cor, P/N: 927-6001), followed by blocking for one hour of blocking in the same buffer. .. Primary antibodies were prepared in Li-Cor Intercept T20 (TBS) Antibody Diluent (Li-Cor, P/N: 927-65001) and after blocking were placed on the membranes overnight on a rocker at 4° C. Primary antibodies used include α-synuclein (Abcam, ab155038) and p16 (Abcam, ab51243).

    Incubation:

    Article Title: A Borophosphate Glass Doped with Cobalt Oxide Improves Skeletal Muscle Structure and Function in Myopathic Mice
    Article Snippet: Samples were loaded on 4–20% gradient Criterion TGX Midi-Protein gels (Bio-Rad, Hercules, CA, USA) for electrophoresis and transferred to LF-PVDF membranes (Millipore, Burlington, MA, USA). .. Following transfer, membranes were blocked in either 5% milk solution or Intercept TBS Blocking Buffer (Li-Cor Biotechnology, Lincoln, NE, USA) for 1 h at room temperature; followed by incubation with primary antibodies overnight at 4 °C. .. Membranes were exposed to either IRDye 800CW Goat anti-Mouse IgG (RRID: AB_621842, Cat.# 926-32210) or IRDye 800CW Goat anti-Rabbit IgG secondary antibodies (RRID: AB_621843, Cat.# 926-32211) (Li-Cor Biotechnology, Lincoln, NE, USA) for 30–60 min depending on the protein of interest.

    Membrane:

    Article Title: AI platform for CRISPR functional mapping and function-based drug design
    Article Snippet: Protein was transferred using Trans-Blot Turbo Mini 0.2 μm PVDF Transfer Packs (BioRad, 1704156). .. The PVDF membrane was blocked for 40 minutes with a 1:1 ratio of 1x TBS (ThermoFisher, PI28360) and Intercept TBS Blocking Buffer (LICOR, 927-60001). .. After blocking, 1μg/mL of NLRP3 (Invitrogen, PA579740) and 1:6000 GAPDH (Cell Signaling Technologies, 2118) antibody was added and kept at 4C overnight with gentle shaking.

    SDS Page:

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [ ], anti-SODB (1:500) [ ], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [ ] antibodies diluted in TBS Blocking Buffer (LI-COR Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Western Blot:

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [ ], anti-SODB (1:500) [ ], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [ ] antibodies diluted in TBS Blocking Buffer (LI-COR Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Control:

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [ ], anti-SODB (1:500) [ ], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [ ] antibodies diluted in TBS Blocking Buffer (LI-COR Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).

    Article Title: Glycosomal Aquaglyceroporin 1 dual role in iron homeostasis and antimony susceptibility in Leishmania amazonensis.
    Article Snippet: .. Equal protein amounts (20 μg per lane) were resolved by SDS-PAGE and analyzed by western blot using anti-SODA (1:500) [50], anti-SODB (1:500) [50], anti-FLAG M2 (1:4,000, for Cas9 detection; F1804-MG, Sigma-Aldrich), anti-alpha Tubulin (1:5,000, loading control; 62204, Thermo Fisher) and anti-arginase (1:500, loading control) [21] antibodies diluted in TBS Blocking Buffer (LI-COR PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0014141 April 2, 2026 14 / 19 Bioscience). .. After primary incubation, membranes were washed three times in TBST (TBS, 0.1% Tween-20) and incubated for 1 h at room temperature, protected from light, with IRDye 800CW goat anti-Rabbit or IRDye 680RD anti-Mouse (LICORbio; 1:10,000 in TBS Blocking Buffer).



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